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Sanitation installation and gas installation in multifunctional building
Vaněk, Ondřej ; Bárta,, Ladislav (referee) ; Vrána, Jakub (advisor)
Bachelor Thesis ,,Technical and gas installations inmulti-building‘‘ is processed in the form of project documentationplumbing and gas installations for building construction in accordance with applicable regulations. Two-storey building is designed, besement. The building is used on the first floor of the municipal office and on the second floor of meeting andbanqueting facilities. Project part focuses on the design and solvingplumbing and gas installations. Part of the thesis are the total property assessmentsand literature review focused on the issue of hot water.
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Pepper quality in terms of piperine content
Lamplot, Marek ; Vítová, Eva (referee) ; Diviš, Pavel (advisor)
The aim of this bachelor's thesis was to determine the quality of pepper available in the commercial network of the Czech Republic. The quality of the pepper samples was determined based on the piperine content. Piperine was extracted from pepper samples using acetone and the extracts were subsequently analyzed using high-performance liquid chromatography with a mass detector. In all samples, the water content was determined by Karl-Fisher titration, and the piperine content in the samples was subsequently converted to dry matter. The content of piperine in pepper varied from 49 to 90 mg/g and met the requirements of the current legislation, which specifies a minimum piperine content of 4 % in pepper. Statistical analysis showed that there is a statistically significant difference between individual pepper samples in terms of piperine content, which points to the fact that pepper from different producers comes from different sources.
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Interaction of selected natural substances used in food industry with DNA and its structural motifs
Gardošová, Zuzana ; Pernicová, Iva (referee) ; Brázda, Václav (advisor)
G-quadruplexes represent secondary DNA structures formed in guanine-rich nucleic acid regions. These structures are involved in many biological processes, including DNA replication, transcription, and telomere maintenance. Several natural substances interacting with G-quadruplex structures have been described. Many of them can be used in the treatment of cancer or other areas of therapeutic practice. G-quadruplexes are dynamic structures whose stability can be affected by a variety of different factors, including chemical modifications to DNA. One of these modifications is DNA methylation, which is an important epigenetic mechanism regulating gene expression. DNA methylation can affect the function and stability of G-quadruplex structures. The theoretical part of the present work focuses on DNA secondary structures, characterization of G-quadruplexes and their ligands and describes the relationship between DNA methylation and G-quadruplex structures. In the experimental part, the binding ability of the natural substances quercetin, berberine, piperine, and caffeine to G-quadruplex structures formed in telomeric oligonucleotide sequences and sequences derived from the proto- oncogene c-Myc was confirmed. Furthermore, the ability of berberine and quercetin was proven to stabilize G-quadruplexes in the aforementioned sequences. Bioinformatics analysis showed that the frequency of G4 is higher in CpG regions than in their surroundings, and the highest frequency of G4 within CpG regions was observed on chromosome 19. Global methylation assays demonstrated that the breast cancer cell line exhibited hypomethylation compared to the non-tumor human dermal fibroblast cell line. After treatment with berberine, the analyzed DNA of both cell lines showed hypermethylation, whereas DNA after interaction with quercetin showed hypomethylation.
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Sanitation installation and gas installation in multifunctional building
Vaněk, Ondřej ; Bárta,, Ladislav (referee) ; Vrána, Jakub (advisor)
Bachelor Thesis ,,Technical and gas installations inmulti-building‘‘ is processed in the form of project documentationplumbing and gas installations for building construction in accordance with applicable regulations. Two-storey building is designed, besement. The building is used on the first floor of the municipal office and on the second floor of meeting andbanqueting facilities. Project part focuses on the design and solvingplumbing and gas installations. Part of the thesis are the total property assessmentsand literature review focused on the issue of hot water.
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